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Image Search Results
Journal: Leukemia
Article Title: Synaptojanin 2 is recognized by HLA class II-restricted hairy cell leukemia-specific T cells.
doi: 10.1038/sj.leu.2403174
Figure Lengend Snippet: Figure 3 Syn 2 expression was determined by Western blot analysis. For each sample protein extracts were made of 5 106 cells, of which 25 mg of total protein was separated on a 6.5% denaturating SDS-PAGE gel. Next, proteins were blotted on nitrocellulose membranes and the presence of Syn 2 was shown using specific polyclonal antibodies.21 (a) Syn 2 expression in HCL material of 12 different HCL patients. (b) Syn 2 expression in various cell lines and hematologic malignancies. Lane 1, Hela; lane 2, Molt-4; lane 3, K562; lane 4, AML 193; lane 5, U 937; lane 6, HCL pat A; lane 7, EBV-LCL pat A; lane 8, HCL pat B; lane 9, EBV-LCL pat B; lane 10, B-ALL pat A; lane 11, B-ALL pat B; lane 12, B-NHL pat A; lane 13, B-NHL pat B; lane 14 AML pat A. For cell line AML193 also smaller protein fragments could be detected, probably protein products of alterna- tively spliced Syn 2.
Article Snippet: The human myelomonocytic leukemia cell line U937 (ATCC CRL-1593.2), the acute
Techniques: Expressing, Western Blot, SDS Page
Journal: Leukemia
Article Title: Synaptojanin 2 is recognized by HLA class II-restricted hairy cell leukemia-specific T cells.
doi: 10.1038/sj.leu.2403174
Figure Lengend Snippet: Figure 4 Recognition of AML193 by clone Vb8S3 þ after transduction with HLA DPw4 Clone Vb8S3 þ was incubated for 3 days with wild-type AML-193, AML-193 mock transfected, two AML- 193 lines transduced with HLA DPA1n0103 and HLA DPBn0401 (both transduced lines were made independently). The proliferation of the T cells alone were shown as a control. The open bars represent background proliferation (3H-thymidine incorporation) given by the irradiated target cells. The hatched bars show proliferation of clone Vb8S3 þ after preincubation with the target cells for 3 days.
Article Snippet: The human myelomonocytic leukemia cell line U937 (ATCC CRL-1593.2), the acute
Techniques: Transduction, Incubation, Transfection, Control, Irradiation